Sunday, December 7, 2014

The Purpose (Protein)- Translation


Act I: Initiation:
The large and small ribososomal subunits binds to the 5' cap of a mRNA molecule and scans it until it reaches the AUG start codon (which corresponds to the amino acid methionine). Then the 1st aminoacyl-tRNA(the indicator methionine-tRNA) binds to the start codon in the P site.
Act II: Elongation:
1. Another tRNA with  an anticodon and amino acid that corresponds with the codon (a threesome of bases on the mRNA) in the A site enters the ribosome. Next the animo acid (Met),  from the tRNA in the P site froms a peptide bond with the amino acid on the tRNA in the A site.
The Wobble effect is when even if the third base of a codon is different, it still codes for the same amino acid, thus reducing the chance of mutation.
2. During translocation, the ribosome moves to the next codon while the 2 tRNA remain bound to their codons, leaving a vacant A site, and the empty tRNA in the E site (where tRNA is released).
Steps 1&2 are repeated for each codon.
Act II: Termination:
When the A site arrives at a stop codon (UAA, UAG, or UGA), a protein release factor binds instead of a tRNA. The polypeptide chain is released from the tRNA, because there is no amino acid in the A site. The ribosomal units separate and the tRNA and the release factor are also freed.

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